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pher3 tyr1289  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc pher3 tyr1289
    Pher3 Tyr1289, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pher3+tyr1289/Phospho-HER3%2FErbB3+(Tyr1289)+Rabbit+mAb/10__1158_slash_1535___7163__mct___25___0505-98-52-54
    Average 95 stars, based on 320 article reviews
    pher3 tyr1289 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Western Blot:

    Article Title: HER3 targeting with an antibody‐drug conjugate bypasses resistance to anti‐HER2 therapies
    Article Snippet: GAPDH Western, 1:10,000 sc-166574 Santa Cruz Biotechnol. .. Cleaved caspase 3 Western, 1:1,000 #9664 Cell Signaling Technol. pHER2 (Tyr1221/22) Western, 1:1,000 #2249 Cell Signaling Technol. pHER3 (Tyr1289) Western, 1:5,000 #4791 Cell Signaling Technol. pHistone H3 (Ser10) Western, 1:6,000 06-570 Merck HER2 (Ab3) Western, 1:10,000 OP15 Calbiochem Calnexin Western, 1:40,000 ADI-SPA-860-F Enzo Life Science BubR1 Western, 1:3,500 612503 BD Biosciences pAKT (pS473) Western, 1:5,000 560397 BD Biosciences HER3 Western, 1:5,000 Made in house Sanchez-Martin, 2012 Rabbit Ig (HRP) Western, 1:20,000 1706515 Bio Rad Laboratories Mouse Ig (Starbright 520) Western, 1:5,000 12005867 Bio Rad Laboratories Rabbit Ig (Starbright 700) Western, 1:5,000 12004162 Bio Rad Laboratories Human Ig (HRP) Western, 1:40,000 109-035-088 Jackson Immunores. .. Mouse Ig (HRP) Western, 1:10,000 NA931 GE Healthcare β-tubulin Immunofluorescence, 1:200 T4026 Sigma-Aldrich LAMP1 Immunofluorescence, 1:100 #9091 Cell Signaling Technol.

    Article Title: Targeting tumor microenvironment-derived NRG1-HER2/3 signaling with zenocutuzumab restores sensitivity to AR inhibition in PTEN wild-type prostate cancer
    Article Snippet: .. The following antibodies were used for western blotting and IHC staining: pAKT (Ser473) (Cell Signaling Technology, 4060L, RRID:AB_2315049), pAKT (Thr308) (Cell Signaling Technology, 4056S, RRID:AB_331163), pS6 (Ser235/236) (Cell Signaling Technology, 4856S, RRID:AB_2181037), pPRAS40 (Thr246) (Cell Signaling Technology, 2997S, RRID:AB_2258110), HSP90 (Cell Signaling Technology, 4875S, RRID:AB_2233331), pHER2 (Tyr1221/1222) (Cell Signaling Technology, 2243, RRID:AB_490899), pHER3 (Tyr1289) (Cell Signaling Technology, 4791, RRID:AB_2099709), STEAP1 (Santa Cruz Biotechnology, SC271872, RRID:AB_10707830), PMEPA1 (Santa Cruz Biotechnology, SC-293372, RRID:AB_3717318), FKBP5 (Cell Signaling Technology, 8245, RRID:AB_10831198), AR (Abcam, ab108341, RRID:AB_10865716), PTEN (Cell Signaling Technology, 9559, RRID:AB_390810), COL1A2 (Proteintech, 66761-1-IG, RRID:AB_2882107). .. The following cytokines were used for stimulation experiments to assess cell growth or protein expression: Recombinant Human Heregulinβ1 (NRG1) (PeproTech, 100-03), Recombinant Human Amphiregulin (PeproTech, 100-55B).

    other:

    Article Title: Biological materials related to HER3
    Article Snippet: Cells were stimulated with 100 ng/ml HRG1-β1 EGF domain (R&D Systems, #396-HB) for 10 min, supernatants discarded and cells lysed in cold RIPA lysis buffer (1% NP-40 (Nonidet P40), 0.5% (V/V) Sodium-Deoxycholat, 0.1% (V/V) SDS, 50 mM Tris/HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, protease inhibitor cocktail set III (Calbiochem), phosphatase inhibitor cocktail set II (Calbiochem)) for 45 min at 4° C. After centrifugation, 4× sample buffer (Biorad #161-0791) was added to the supernatants and samples analysed by SDS-PAGE (Criterion XT precast get, 4-12%, Bis Tris, Biorad #345-0125) followed by WB.

    Saline:

    Article Title: A Potent HER3 Monoclonal Antibody That Blocks Both Ligand-Dependent and -Independent Activities: Differential Impacts of PTEN Status on Tumor Response
    Article Snippet: After a brief incubation, cell lysates were collected, equal amounts were loaded onto Bis NuPAGE Novex Bis-Tris gels (Invitrogen) and proteins transferred onto polyvinylidene fluoride (PVDF) membranes (Invitrogen). .. Membranes were blocked with 5% nonfat dry milk and 0.1% Tween 20 (Sigma) in Tris-buffered saline pH 7.4 (TBS) and incubated overnight at 4°C with antibodies to pHER3-Tyr1289 (4791, Cell Signaling), pAKT-Ser473 (4060, Cell Signaling), and neuregulin-1/HRG (NRG1/HRG) antibody (sc-348, Santa Cruz). .. An antibody to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (G8795, Sigma) was used to ensure equal amount of protein was loaded across all wells.

    Incubation:

    Article Title: A Potent HER3 Monoclonal Antibody That Blocks Both Ligand-Dependent and -Independent Activities: Differential Impacts of PTEN Status on Tumor Response
    Article Snippet: After a brief incubation, cell lysates were collected, equal amounts were loaded onto Bis NuPAGE Novex Bis-Tris gels (Invitrogen) and proteins transferred onto polyvinylidene fluoride (PVDF) membranes (Invitrogen). .. Membranes were blocked with 5% nonfat dry milk and 0.1% Tween 20 (Sigma) in Tris-buffered saline pH 7.4 (TBS) and incubated overnight at 4°C with antibodies to pHER3-Tyr1289 (4791, Cell Signaling), pAKT-Ser473 (4060, Cell Signaling), and neuregulin-1/HRG (NRG1/HRG) antibody (sc-348, Santa Cruz). .. An antibody to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (G8795, Sigma) was used to ensure equal amount of protein was loaded across all wells.

    Immunohistochemistry:

    Article Title: Targeting tumor microenvironment-derived NRG1-HER2/3 signaling with zenocutuzumab restores sensitivity to AR inhibition in PTEN wild-type prostate cancer
    Article Snippet: .. The following antibodies were used for western blotting and IHC staining: pAKT (Ser473) (Cell Signaling Technology, 4060L, RRID:AB_2315049), pAKT (Thr308) (Cell Signaling Technology, 4056S, RRID:AB_331163), pS6 (Ser235/236) (Cell Signaling Technology, 4856S, RRID:AB_2181037), pPRAS40 (Thr246) (Cell Signaling Technology, 2997S, RRID:AB_2258110), HSP90 (Cell Signaling Technology, 4875S, RRID:AB_2233331), pHER2 (Tyr1221/1222) (Cell Signaling Technology, 2243, RRID:AB_490899), pHER3 (Tyr1289) (Cell Signaling Technology, 4791, RRID:AB_2099709), STEAP1 (Santa Cruz Biotechnology, SC271872, RRID:AB_10707830), PMEPA1 (Santa Cruz Biotechnology, SC-293372, RRID:AB_3717318), FKBP5 (Cell Signaling Technology, 8245, RRID:AB_10831198), AR (Abcam, ab108341, RRID:AB_10865716), PTEN (Cell Signaling Technology, 9559, RRID:AB_390810), COL1A2 (Proteintech, 66761-1-IG, RRID:AB_2882107). .. The following cytokines were used for stimulation experiments to assess cell growth or protein expression: Recombinant Human Heregulinβ1 (NRG1) (PeproTech, 100-03), Recombinant Human Amphiregulin (PeproTech, 100-55B).

    Staining:

    Article Title: Targeting tumor microenvironment-derived NRG1-HER2/3 signaling with zenocutuzumab restores sensitivity to AR inhibition in PTEN wild-type prostate cancer
    Article Snippet: .. The following antibodies were used for western blotting and IHC staining: pAKT (Ser473) (Cell Signaling Technology, 4060L, RRID:AB_2315049), pAKT (Thr308) (Cell Signaling Technology, 4056S, RRID:AB_331163), pS6 (Ser235/236) (Cell Signaling Technology, 4856S, RRID:AB_2181037), pPRAS40 (Thr246) (Cell Signaling Technology, 2997S, RRID:AB_2258110), HSP90 (Cell Signaling Technology, 4875S, RRID:AB_2233331), pHER2 (Tyr1221/1222) (Cell Signaling Technology, 2243, RRID:AB_490899), pHER3 (Tyr1289) (Cell Signaling Technology, 4791, RRID:AB_2099709), STEAP1 (Santa Cruz Biotechnology, SC271872, RRID:AB_10707830), PMEPA1 (Santa Cruz Biotechnology, SC-293372, RRID:AB_3717318), FKBP5 (Cell Signaling Technology, 8245, RRID:AB_10831198), AR (Abcam, ab108341, RRID:AB_10865716), PTEN (Cell Signaling Technology, 9559, RRID:AB_390810), COL1A2 (Proteintech, 66761-1-IG, RRID:AB_2882107). .. The following cytokines were used for stimulation experiments to assess cell growth or protein expression: Recombinant Human Heregulinβ1 (NRG1) (PeproTech, 100-03), Recombinant Human Amphiregulin (PeproTech, 100-55B).



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