pher3 tyr1289 (Cell Signaling Technology Inc)
95
Structured Review
Cell Signaling Technology Inc
pher3 tyr1289
Pher3 Tyr1289, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pher3+tyr1289/Phospho-HER3%2FErbB3+(Tyr1289)+Rabbit+mAb/10__1158_slash_1535___7163__mct___25___0505-98-52-54
Average 95 stars, based on 320 article reviews
Pher3 Tyr1289, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pher3+tyr1289/Phospho-HER3%2FErbB3+(Tyr1289)+Rabbit+mAb/10__1158_slash_1535___7163__mct___25___0505-98-52-54
Average 95 stars, based on 320 article reviews
pher3 tyr1289 - by Bioz Stars,
2026-09
95/100 stars
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Western Blot:Article Title: HER3 targeting with an antibody‐drug conjugate bypasses resistance to anti‐HER2 therapies Article Snippet: GAPDH Western, 1:10,000 sc-166574 Santa Cruz Biotechnol. .. Cleaved caspase 3 Western, 1:1,000 #9664 Cell Signaling Technol. pHER2 (Tyr1221/22) Western, 1:1,000 # Article Title: Targeting tumor microenvironment-derived NRG1-HER2/3 signaling with zenocutuzumab restores sensitivity to AR inhibition in PTEN wild-type prostate cancer Article Snippet: .. The following antibodies were used for western blotting and IHC staining: pAKT (Ser473) (Cell Signaling Technology, 4060L, RRID:AB_2315049), pAKT (Thr308) (Cell Signaling Technology, 4056S, RRID:AB_331163), pS6 (Ser235/236) (Cell Signaling Technology, 4856S, RRID:AB_2181037), pPRAS40 (Thr246) (Cell Signaling Technology, 2997S, RRID:AB_2258110), HSP90 (Cell Signaling Technology, 4875S, RRID:AB_2233331), pHER2 (Tyr1221/1222) (Cell Signaling Technology, 2243, RRID:AB_490899), other:Article Title: Biological materials related to HER3 Article Snippet: Cells were stimulated with 100 ng/ml HRG1-β1 EGF domain (R&D Systems, #396-HB) for 10 min, supernatants discarded and cells lysed in cold RIPA lysis buffer (1% NP-40 (Nonidet P40), 0.5% (V/V) Sodium-Deoxycholat, 0.1% (V/V) SDS, 50 mM Tris/HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, protease inhibitor cocktail set III (Calbiochem), phosphatase inhibitor cocktail set II (Calbiochem)) for 45 min at 4° C. After centrifugation, 4× sample buffer (Biorad #161-0791) was added to the supernatants and samples analysed by SDS-PAGE (Criterion XT precast get, 4-12%, Bis Tris, Biorad #345-0125) followed by WB. Saline:Article Title: A Potent HER3 Monoclonal Antibody That Blocks Both Ligand-Dependent and -Independent Activities: Differential Impacts of PTEN Status on Tumor Response Article Snippet: After a brief incubation, cell lysates were collected, equal amounts were loaded onto Bis NuPAGE Novex Bis-Tris gels (Invitrogen) and proteins transferred onto polyvinylidene fluoride (PVDF) membranes (Invitrogen). .. Membranes were blocked with 5% nonfat dry milk and 0.1% Tween 20 (Sigma) in Tris-buffered saline pH 7.4 (TBS) and incubated overnight at 4°C with antibodies to Incubation:Article Title: A Potent HER3 Monoclonal Antibody That Blocks Both Ligand-Dependent and -Independent Activities: Differential Impacts of PTEN Status on Tumor Response Article Snippet: After a brief incubation, cell lysates were collected, equal amounts were loaded onto Bis NuPAGE Novex Bis-Tris gels (Invitrogen) and proteins transferred onto polyvinylidene fluoride (PVDF) membranes (Invitrogen). .. Membranes were blocked with 5% nonfat dry milk and 0.1% Tween 20 (Sigma) in Tris-buffered saline pH 7.4 (TBS) and incubated overnight at 4°C with antibodies to Immunohistochemistry:Article Title: Targeting tumor microenvironment-derived NRG1-HER2/3 signaling with zenocutuzumab restores sensitivity to AR inhibition in PTEN wild-type prostate cancer Article Snippet: .. The following antibodies were used for western blotting and IHC staining: pAKT (Ser473) (Cell Signaling Technology, 4060L, RRID:AB_2315049), pAKT (Thr308) (Cell Signaling Technology, 4056S, RRID:AB_331163), pS6 (Ser235/236) (Cell Signaling Technology, 4856S, RRID:AB_2181037), pPRAS40 (Thr246) (Cell Signaling Technology, 2997S, RRID:AB_2258110), HSP90 (Cell Signaling Technology, 4875S, RRID:AB_2233331), pHER2 (Tyr1221/1222) (Cell Signaling Technology, 2243, RRID:AB_490899), Staining:Article Title: Targeting tumor microenvironment-derived NRG1-HER2/3 signaling with zenocutuzumab restores sensitivity to AR inhibition in PTEN wild-type prostate cancer Article Snippet: .. The following antibodies were used for western blotting and IHC staining: pAKT (Ser473) (Cell Signaling Technology, 4060L, RRID:AB_2315049), pAKT (Thr308) (Cell Signaling Technology, 4056S, RRID:AB_331163), pS6 (Ser235/236) (Cell Signaling Technology, 4856S, RRID:AB_2181037), pPRAS40 (Thr246) (Cell Signaling Technology, 2997S, RRID:AB_2258110), HSP90 (Cell Signaling Technology, 4875S, RRID:AB_2233331), pHER2 (Tyr1221/1222) (Cell Signaling Technology, 2243, RRID:AB_490899), |